FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Neurotransmitter Transporter Uptake Assay Kit. The experiment was performed in 4 technical replicates (wells in the cell culture plate). In addition, the instrument features linear and orbital shaking, plus temperature control. Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. Catalog. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 3 Pages. The Evans blue dye was extracted from the ears with 1 ml of formamide overnight at 55°C and measured spectrophotometrically at 620 nm (FilterMax TM F5 Multi-Mode Microplate Reader). Fluorescence polarization was measured at 21°C using FilterMax™ F5 Multimode Microplate Reader (Molecular Devices) with excitation and emission wavelengths of 485 nm and 535 nm, respectively. 005 OD. Configurability is an important aspect of BioTek’s multi-mode readers to provide the most value for laboratory budgets, but the independent optical systems in our multi-mode readers don’t compromise on performance. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Learn how to set up protocols, customize reports, export results, and more with this PDF document. The ucs load command creates a backup of. ScanLater Western Blot Detection System. 00 USD. There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. d. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. 2. Devices FilterMax Multi-Mode Microplate Readers. Method Fluorescence intensity Read Type Endpoint / Kinetic Optics Type Filters Step Label "Linearity Read" Read well C1-F5 Filter Set. A) CHO colonies, serum-free suspension-adapted, in CloneMedia-CHO. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $3,250. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). Reaction was stopped by the addition of 100 μl Stop Solution and optical density of each well was determined using a FilterMax F5 Multi-Mode microplate reader and SoftMax Pro 6. Optical densities (OD) from ELISA assays were measured with the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA). 40266 Email: drugdiscoverytech@lifetech. A microplate reader is laboratory machine used for the estimation of biochemical or. Transfluor Technology. The OD 600 was plotted against time using Graphpad Prism 8. Cytokine concentration was determined by plotting the OD or median fluorescence intensity (MFI) of each sample on each cytokine concentration curve and multiplying the result by a dilution. The other members of the E39 family (525i, 530i, and 540i) do not have limited slip. The FlexStation 3 Benchtop Multi-Mode Microplate Reader can measure absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence. Multi-Mode Microplate Reader. They are very complex instruments. The MTT assay was performed with 100 μM vitamin E in the presence or absence of LPS at a concentration of 1 µg/mL for 24 h. At day 4 P. 00. Devices FilterMax Multi-Mode Microplate Readers. The OD 600 was plotted against time using Graphpad Prism 8. Users can program endpoint, kinetic, multiple wavelength, linear scan and area scan measurement types. Add to my manuals. F5 has made an exception to this policy for BIG-IP 13. Enzymatic activity was calculated after subtracting absorbance decay obtained from buffer without cell lysates by using NADPH extinction coefficient of 6220/M/cm and by. SpectraMax Glo Steady-Luc Reporter Assay Kit. FilterMax F3/F5 Multi- Mode Microplate Readers · FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: of 6 /6. Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader. The increase in NADH, an end product in this reaction, was determined by means of its light absorbance at 355 nm using a fluorometric microplate reader (FilterMax F5 Multi-Mode Microplate Reader; Molecular Devices,. SpectraMax M-series Microplate readers. Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat SYRINGE/VALVE CHAMBER FOR RQF INSTRUMENT quench flow analysis KIN-TEK RAPID QUENCH FLOW INSTRUMENT KIN-TEK RQF-3 VALVE/SYRINGE CHAMBER Biorad Laboratory Real Time PCR System& FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. Match case Limit results 1 per page. SpectraMax iD3 and iD5 Multi-Mode Microplate Readers;. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. ImageXpress Micro 4 High-Content Imaging System. $3,000 USD. Triplicates of. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The standard curve presented a linear phase from 1. FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. A microplate consists of small wells wherein separate reactions take place. Absorbance at 570 nm was recorded on a CLARIOstar multi-mode microplate reader (BMG Labtech, Germany). 00 USD. Important things to consider when purchasing a multimode microplate reader. F5 Multi-Mode Microplate Reader. Readers ®Examples: Monochromators such as the SpectraMax i3 Multi-Mode Microplate Reader Detection Platform or filter based systems like the FilterMax™ F3 and F5 Multi-Mode Microplate Readers Luminescence Principle The total amount of light emitted is collected Plate specs White plates to reflect all the light to the detectorSpectraMax i3/i3x Multi-Mode Microplate Reader HeLa cells Cell viability assay SoftMax Pro Software Simplified homogenous experimental protocol The EarlyTox cell viability assay kits apply a no-wash, homogeneous protocol that simplifies the workflow and reduces the data variability typically encountered with multiple experimental steps. Molecular Devices, LLC Reading Modes: Endpoint, Kinetic, Linear Scan, Area Scan; Optical System: LEDs, Deuterium Lamp; Wavelength Range: 230 to 750 nm;. com 8. Match case Limit results 1 per page. org) with optimized instrument settings b. Absorbance was measured at 450 nm using a FilterMAx F5 Multi-Mode Microplate Reader and EX2 filter after a 2-hour incubation at 37°C with 10 μL of CCK-8 per 100 μL of media. Caspase-3 Activity. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. #0200-7030) Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. Set up plate with cells, reagent and test compounds. In the list below the slide number 21, select the positions where the emission filters are installedF3/F5 Multi-Mode Microplate Readers. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. #6590-0121)The MTT solution was withdrawn, and 200 µl of dimethyl sulphoxide (DMSO) was added to determine the absorbance values, at an absorption curve of λ= 595 nm (FilterMax F5 Multi-Mode Microplate reader, Molecular Probes). Cytokines Assay. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). Upgradeable modules are also available including western blot, cell imaging, and fast. Manufacturer: Molecular Devices Model: FilterMax F5 Condition: Used. 3 Pages. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceF3/F5 Multi-Mode Microplate Readers. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). You can also contact Technical Support by email. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seEye lysates were developed using a Glucose Colorimetric Assay Kit (Sigma, Louis, MO, USA) and measured by a FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 570 nm. 40266 Email: drugdiscoverytech@lifetech. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. 2 μM ATP standard was. The microplates were incubated at 37°C for 24 h, and the growth of bacteria was determined by measuring the optical density (OD) at 630 nm using an ELISA microplate reader (FilterMax F5 multi-mode microplate reader, Molecular Devices, Sunnyvale, CA, USA). The capability of the lixiviated products of the experimental membranes to inhibit the bacterial planktonic growth was estimated from the turbidity of the inoculated culture media at OD λ = 595 nm (filter max F5 multi-mode microplate reader). MIA PaCa-2 cells were cultured in 96-well plates with 1000 cells/well. The microplate drawer door must be held open manually while. The absorption was measured in a spectrophotometer ultraviolet–visible (UV–Vis) Filter Max F5 multi-mode microplate reader; a wavelength of 595 nm was taken as the standard value for bacteria. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. BMG Labtech - FLUOSTAR OMEGA. The absorbance was measured at 450 nm using a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. Vascular. the luminescence reading was taken on a FilterMax F5 Multi-Mode. Statistical analysis was performed using. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. MetaFluor Software. Fluorescence kinetics were monitored from the top in a FilterMax F5 Multi-Mode Microplate Reader and the Multi-Mode Analysis Software 3. 6 to 25 ng of prokaryotic recombinant protein reaching a plateau in concentrations above 25 ng. Consult Molecular Devices's entire F3/F5 Multi-Mode Microplate Readers catalogue on MedicalExpo. The haemolytic activity was determined in the cell supernatant by optical density readings at 570 nm (FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices). Samples (180 μl) were stained with 20 μl of Nile red (2. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. Molecular Devices Microplate reader VersaMax $1,495. FilterMax F5 Multi-Mode Microplate Reader. An MTT assay was performed with 5 mM BHB in the presence or absence of LPS (1 µg/mL) for 24 h. The FilterMax F3 features absorbance (visible), fluorscence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. 50/50. Discover Precision and Reliability with Our Featured General Laboratory Instruments - Thermo Forma 3110 Water Jacketed CO2 Incubator, Thermo ULT185-5-V -80 Freezer, Filtermax F5 Multimode Microplate Reader, Spectramax M5e, Thermo Napco CO2 Incubator, and XS 64 Balance. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 600 of the culture in the plate was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 20 min over 20 h. To do so, perform one of the. CloneMatrix. 9 mM H 2 O 2 for. The OD value of each tube (230 μL) was measured in a 96-well microplate at 405 nm using a. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. 4 Pages. In addition, the FilterMax F5 Reader is capable of measuring. After incubation, each microplate was analyzed for absorbance reading. 5 min for 30 min at 340 nm absorbance using a microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, FilterMax F5 Multi-Mode Microplate Reader) and Multi-Mode Analysis Software (Molecular Devices, RRID:SCR_014789), version 3. com A. 5 mP. Datapaq Express (Vial Rack Bottom Label 2D Reader) XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. Click on the microplate icon in the upper left corner and then click "New" to open a new data file: In the section "Plate1", click on the Settings icon. F3/F5 Multi-Mode Microplate Readers Fluorescent signals from the same assay plate were measured on the SpectraMax iD5 reader (the SpectraMax iD3 reader is also suitable for these assays, as it has the same onboard fluorescence performance as the SpectraMax iD5 reader), SpectraMax i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. The measured temperature can be used to provide feedback. Your applications, your modes, your choice. ** Some compatible filter sets are available for purchase from Life. Transfluor Technology. FAST AND ACCURATE METERTECH Microplate Reader $3,650. At least three independent experiments were performed for each. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. SpectraMax® M5/M5e Microplate Reader & FlexStation® 3 Benchtop Multi-Mode Microplate ReaderSpectraMax M-series Microplate readers. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 1. Supplier: Molecular Devices. Luminescence was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate ReaderThe upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. Luminescence (glow) Fluorescence polarization. Add to cart. , call 1-800-635-5577. *** (p < 0. The amount of each HTRA1 protein was. PerkinElmer Multimode Plate Reader EnSpire 2300 $8,495. USD ($) USD - United States Dollar. BMG POLARstar Microplate Reader, 115-230V, BMG Labtech (CAT#: STEM-SPO-0061-YJL) Perkin Elmer Victor3 1420 Multilabel Counter, 110-240V, 3 Amps, PerkinElmer (CAT#: STEM-SPO. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. The planktonic growth inhibition (%) was calculated according to the following equation:Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. 4 Pages. FilterMax® F3 & F5 Multi-Mode Microplate Readers. The following multi-step binary elution gradient, based on an water as phase A and acetonitrile as phase B, both containing 0. The plates were loaded into a FilterMax TM F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA), and the fluorescence intensity was measured at 37 °C with Ex/Em = 440/484 nm and 15 s shaking between reads to facilitate aggregation. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry. The values of luminescence are directly proportional to the number of viable cells. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. The drawing shows a standard microplate of 15 mm height covered with a lid. 3 Pages. Hit enter to search or ESC to close. 7°C (gradient heating design). Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). Cell growth and viability were assessed using the CCK-8 proliferation assay. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. MetaMorph NX. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence. Absorbance values recorded at 490 nm using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices) were then analyzed with Excel software (Office, Microsoft) and GraphPad Prism 6 software (La Jolla, CA, USA) to assess statistical significance. 7. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. J. Microplate Readers. 9. 4 Pages. 4. After further centrifugation in the same fashion, the supernatant was subjected to subsequent enzymatic reactions. 100 fmol 384-well (75 µL) 0. For FcγRI (high affinity receptor) binding assays, plates were coated with 1 μg/mL recombinant human soluble. An anti-human HER2/neu human IgG3/κ (Huang and Morrison, 2006) was used as isotype (negative) control. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. The samples were transferred to black 96-well plates and fluorescence was measured at an excitation wavelength of 405 nm and an emission wavelength of 535 nm on the FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices) equipped with the SoftMaxPro Microplate Data Acquisition and Analysis 6. Please click on the links below to view the Microplate Reader Comparison Tables. BioStack Neo is a dedicated stacker offering super-FilterMax™ F3 and FilterMax™ F5 Multi-Mode Microplate Reader User Guide 5008528 A September 2010 DTX Multimode Readers User Guide. • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. The inter-assay coefficient of variations (CV) and values for all biomarkers can be found in Table 4. , Sunnyvale, CA, USA) and normalized to the. shopping cart 0 items $0. 8 Pages. GPCRs. 8 Pages. 9). & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. Readings at 575 nm were subtracted from the readings at 450 nm to correct for optical imperfections in the plate. Plaque reduction. FilterMax F5 Multi-Mode Microplate Reader. Technical specifications Compatible with these Molecular Devices systems Contact Us Phone: +1-800-635-5577 Email: [email protected]. Fluorescence intensity was determined using a FilterMax F5 multi-mode microplate reader at an excitation wavelength of 485 and an emission wavelength of 530nm. To contact technical support by phone in the U. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. Luminoskan luminometric microplate reader. Click to Contact Seller. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. You can determine the model and type of your F5 hardware platform through the command line or the Configuration utility. Search LabX. LabX is a multi-vendor marketplace that connects you with the life science tools and chemistry you need to run your lab. This minor release branch supports the B2100 (A109) hardware product until the product's End of Software. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. FilterMax F5 Multi-mode microplate reader. Besides the number of substrates used by microbiota,. 5. 012 fmol 0. Lonza Luminometers Instrument MycoAlert™ Assay MycoAlert™ PLUS Assay Other Bioassays* Notes Lonza Lucetta™ Luminometer + + Cuvette Tailor -made MycoAlert™ Mode Luminometers from Other SuppliersTriplicate reactions were measured every 2. Related Products: FilterMax F3 &. ; Page 2 For research use only. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. 97059-524EA 84727. com A. FilterMax F5 Multi-mode microplate reader. The absorbance values of the results were expressed as optical density (OD) as the mean ± standard deviation. F3/F5 Multi-Mode Microplate Readers. F3/F5 Multi-Mode Microplate ReadersCell pellets were lysed and HTLV-1 LTR-transactivation was measured via luciferase assay according to the manufacturer’s protocol (Dual-Luciferase ® Reporter Assay System, Promega Corporation, Madison, WI; Filtermax F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA) . 3 Pages. The OD 595 of the culture in the plate (light path length: ∼6. • DNA/RNA quantitation • Reporter gene assay The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536-well plates. SoftMax® Pro Microplate Data Analysis Software provides integrated instrument control and data analysis. Plate Formats:. F3/F5 Multi-Mode Microplate ReadersThe plate was incubated for 1 hr at room temperature with shaking. please click here. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The reactions were analyzed in a fluorometer (F5 Multi-Mode Microplate Reader) at 590 nm after a 120-h incubation. LVF Monochromator™ : Excitation and Emission. , a long-time leader in bioanalytical systems for microplate analysis, today announced the launch of two new multi-mode detection platforms that expand its portfolio of microplate readers, enabling the company to meet all combinations of customer needs now and into the future. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Luciferase activity was quantified by the Dual-Glo Luciferase Assay System (Promega) as per the manufacturer’s instructions on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices). 3 Pages. SpectraMax Gemini XPS/EM Microplate Readers. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. 5. 3. , Sunnyvale, CA, USA). ScanLater Western Blot Detection System. Luminescence (glow) Fluorescence polarization. The results were calculated using a malondialdehyde (MDA) curve and expressed as nmol MDA/mg protein. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. 2 μM ATP standard was. When the user applies a set temperature of 37°C, the top is heated to 37. Excitation Filter – EXP1 F535/25, F485/20, F485/20, F370/TRF. 4 Pages. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. 3 and higher include optimized reader settings, as well as data analysis, for faster results. FilterMax F3/F5 Multi- Mode Microplate Readers · FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: of 6 /6. Cells were seeded in 24-well plates and cultured. For 3D cell proliferation assay, (high concentrations) in 3D cellular proliferation assay, 20, 100 or 500 µg/ ml MG were used. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536. book Page 1 Tuesday, September 7, 2010 6:14 PM. Vertical Jump. The experiment was carried out with four biological replicates and two technical replicates. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. SpectraMax iD5. S. 1. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $ 3,250. Plate Formats:. The 0. Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. Luminescence was recorded with a luminometer (Filter Max F5 Multi-Mode Microplate reader) with SoftMax Pro Easy software, and the NADH/NAD + ratio was calculated by comparing the relative light units. shopping cart 0 items $0. Chemiluminescence emission was measured in real time (every 2 min for 120 min) with a FilterMax F5 Multi-Mode Microplate Reader at 27°C and expressed as area under the curve, as previously described . Click “Eject Plate Carrier. 3 Pages. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. . Delete from my manuals. The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). book Page 1 Tuesday, September 7, 2010 6:14 PM. Read Review (1)With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. A standard curve was created by diluting the ATP standards to a range between 2 × 10 −3 and 20 μM ATP. Transfluor Technology. MetaMorph NX. [The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. After 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). The colorimetric signal development was stopped after 10 minutes. 40266 Email: drugdiscoverytech@lifetech. Measurements of Caspase-3/7 Activity. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. , Sunnyvale, CA, USA) for 30 min and 1 h after dye addition. 3. Click Run. Manufacturer: Molecular Devices. , Sunnyvale, CA, USA) and normalized to the absorbance in the. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Transfluor Technology. 8 Pages. 00 USD. Thereafter, the dye liquid was removed, the formazan was solubilized with dimethyl sulfoxide (DMSO) under continuous agitation for 15 min, and the absorbance of each well was read by the means of a microplate reader (FilterMax F5 Multimode Microplate Reader; Molecular Devices) at a wavelength of 570 µm. 100 fmol 1536-well (8 µL) 0. Page 137 Using the same plate as was used in step 3, pipette 50 µL/well of buffer • into wells C1–F5 and C10–F12. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. 3 Pages. Supplier: Molecular Devices. 11 mg/mL in PBS, 20 h incubation) using the FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices (Sunnyvale, CA, USA), at 570 nm . BIO TEK Synergy? 2 Multi-Detection Microplate Reader with Luminescence and Dual Reagent Dispenser SPECTRA MAX 190 MICROPLATE READER (90V TO 240V) WITH CURRENT BMG FLUOSTAR OPTIMA PLATE READER Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat FilterMax F5 Multi-Mode Microplate Reader. » FilterMax™ F5 Multi-Mode Microplate Reader » SpectraMax L Microplate Luminometer ATP + Luciferin + 0 2 Oxyluciferin + AMP + PPi + CO 2 + Light Mg2+ Luciferase Benefits • Sensitive, reliable detection of mycoplasma contamination. The 50% cytotoxic concentration (CC 50) of F5-2a was calculated. The supernatant was taken and 0. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumFlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell. OMRF research scientist Kumar Subramani reviewed BioTek Synergy H1, stating it is a configurable multi-mode microplate reader with high sensitivity. Related Products: FilterMax F3 & F5 Multi-Mode Microplate Readers, SpectraMax i3 Multi-Mode Platform Microplate Detection System,. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. prt Read the plate and then save the experiment. For customers outside the U. A dual-color pyrometer with a selected wavelength away from that of the laser radiation has been used to monitor the melt pool temperature. Delete from my manuals. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. SpectraMax Glo Steady-Luc Reporter Assay Kit. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 5. Products : General, FilterMax F5 reader Please click on the links below to view the Microplate Reader Comparison Tables. 3 Pages. Measurements of Caspase-3/7 Activity. The concentration was expressed as μmol/mg of total protein, based on the use of a standard curve of chloramine T (0-100 μmol/L). Read Review (1)The mixture were then centrifuged at 1000 g for 5 min. The user guide also covers the software features, the maintenance procedures, and the technical specifications of the readers. 003 fmol 0. The absorbance of samples at 405 nm (A 405) was determined at room temperature at 20 and/or 60 min after addition of substrate using a FilterMax F5 Multi-Mode Microplate reader (Molecular Devices, San Jose, CA). Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . ScanLater Western Blot Detection System. 030 fmol Fluorescence Polarization (FP). Open SoftMax® Pro software. Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. A Filter Max F5 Multi-Mode Microplate Reader was used to measure absorbance at 595 nm. The transmittance at 450 nm was determined by FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA). Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Each condition was performed in. 8 Pages. The mixture was then boiled for 10 min. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. Recommended Optics • Use the touchscreen to easily set up protocols, run experiments or view tutorial videos • Automatically identify filter sets using NFC functionality, simplifying workflows and eliminating confusion • Perform western blot detection • Push data to workstations with QuickSync, eliminating the need to retrieve data from the instrument SpectraMax iD5 Multi-Mode Microplate Reader Five-mode. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 2. Protein contamination can be measured at 280 nm. Sign In Upload. Instrument Configuration: Assay Technology Adapta® & FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell-based application requirements while. CloneMatrix. The plate was incubated for 1 hr at room temperature with shaking. The amount of colorimetric product was continuously monitored for 120 min. Page 2 FilterMax F5 and F3 Multi‐Mode Microplate Readers Unpacking and Setup Guide 7. In addition, the instrument features linear and orbital shaking, plus temperature. The solution was incubated for 15 min and fluorescence was measured on a SpectraMax microplate reader (Molecular Devices). The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. DCF fluorescence was measured by using a microplate reader (FilterMax F5) at maximum excitation and with emission spectra of 485 nm and 535 nm, respectively. 3D cell proliferation assays were conducted using high concentrations to optimize conditions for cell lines in 20, 100 or• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax Mini Multi-Mode Microplate Reader. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. After initial incubation, the lysates were incubated with the caspase-1 substrate Ac-YVAD-pNA at 37 °C. 3 Pages. Laser-aided direct metal deposition of metals and alloys. , Shanghai, China) and microplate reader (FilterMax F5 Multi-Mode Microplate Reader, LLC, Molecular Devices, San Jose, CA, United States) at a wavelength of 450 nm. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $7,900. Neurotransmitter Transporter Uptake Assay Kit. After 24 h, the. Devices FilterMax Multi-Mode Microplate Readers. 3 Pages. A blank sample was prepared by mixing. 2. 2. GenePix 4300/4400 Microarray Scanner. Fluorescence of the organic phase was measured at 515 nm of excitation and 553-nm emission using a fluorimeter (FilterMax F5-Multi-Mode Microplate Reader, Molecular Devices, CA, USA). The medium was then removed and the cells washed once with 200 μl of ice-cold phosphate buffered saline (PBS). 40266 Email: [email protected] or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. With optimized reagents, option to operate the. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. ] Example of document to evaluate if the reader . Statistical analysis was performed using. There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). Table of Contents. Absorbance values were normalized on the control at 24 h and analyzed as being proportional to the number of viable cells. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm.